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* **PUS3:** Moving on to PUS3, this enzyme has a more specific focus. PUS3 primarily targets transfer RNAs (tRNAs). tRNAs are the adaptors that bring amino acids to the ribosome during protein synthesis. PUS3-mediated pseudouridine modifications in tRNAs are essential for their structure and function. These modifications can enhance tRNA stability, improve their ability to interact with the ribosome, and increase the fidelity of translation. In other words, PUS3 ensures that tRNAs are in tip-top shape to deliver the correct amino acids during protein synthesis.